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By Philip S. Mellor, Matthew Baylis, Christopher Hamblin, Charles H. Calisher, Peter P.C. Mertens

African horse disease virus is a double-stranded RNA virus which reasons a non-contagious, infectious arthropod-borne illness of equines and sometimes canine. 9 special, the world over known serotypes of the virus have to this point been pointed out. This publication relies upon the findings of 2 programmes funded via the ecu fee. will probably be of worth not just to the expert examine staff but in addition to veterinary staff facing regulate and to legislators trying to advertise secure overseas circulate of equines. the themes coated comprise state of the art discussions on diagnostics, vaccines, molecular biology, vector reviews, and epidemiology.

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In contrast to the vaccinated group, no boost 36 H. : AHSV in mules and donkeys in antibody titres were detected in sera collected from the second group of mules and donkeys after challenge. This result would suggest that the challenge virus was rapidly neutralised and did not initiate a replicating infection at the site of inoculation or in surrounding tissues. References 1. Baylis M, Hasnaoni H, Bouayoune H, Touti J, Mellor PS (1997) The spacial and seasonal distribution of African horse sickness and its potential Culicoides vectors in Morocco.

K. Clinical, virological and immune responses of normal and immunosuppressed donkeys (Equus asinus africanus) after inoculation with African horse sickness virus O. Fassi-FihrP, M. El Harrak2, and M. M. Fassi-Fehri1 1 Departement de Microbiologie, Immunologie et Maladies Contagieuses, Institut Agronomique et Veterinaire Hassan, Rabat-Instituts, Rabat, Morocco 2 Biopharma, Rabat Akkari, Rabat, Morocco Summary. To elucidate the role that donkeys may play in African horse sickness virus (AHSV) persistence during inter-epizootic periods we looked for clinical signs of infection and studied the viraemia and neutralising antibody kinetics in 3 immunocompetent and 3 immunosuppressed donkeys inoculated with AHSV -4.

0 ml dose of AHSV 4 H1E2Hl containing IOS8 ELDsofml. 0 ml dose by the IV route. 8ELDsofml into a single site on the side of the neck by the SC route. 5 ELDsofml by the IV route. 0 ml dose of homogenised spleen suspension collected post mortem from donkey D2 (Study 1). 3 ELDso/ml and was inoculated into three sites as detailed for the donkeys in Study 1. Sampling All equidae were examined daily and their clinical appearance and rectal temperatures recorded. Heparinised and non-heparinised blood samples were collected on three occasions each week.

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